Antioxidant Activity of Valeriana fauriei Protects against Dexamethasone-Induced Muscle Atrophy

Figure 6

Didrovaltrate (DI) inhibits DEX-induced myotube atrophy in C2C12 cells. The inhibitory effect using iridoids of VF. (a, b) The mRNA expression of muscle atrophy markers, such as Atrogin-1 and Murf1, in C2C12 myotubes. DI was found to be the most effective compound. (c) Immunofluorescence staining for MHC (green) and counterstaining with DAPI (blue) in C2C12 myotubes. (d) The fusion index (%) was calculated. (e) Myotube diameter (μm) was measured. (f) The distribution (%) of myotube diameter (μm). (g) The mRNA expression of Mstn in C2C12 myotubes. (h) Representative images of oxidized DCF-DA in C2C12 myoblasts treated with DEX or/and DI. (i) ROS production was measured by DCF fluorescence intensity in C2C12 myoblasts. (j) GR and FOXO3a were detected by western blot analysis in C2C12 myotube cytosol and nuclear fractions, respectively. The data are presented as the . Statistical significance was determined by one-way ANOVA. , versus the nontreated group.

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