IP-Se-06, a Selenylated Imidazo[1,2-a]pyridine, Modulates Intracellular Redox State and Causes Akt/mTOR/HIF-1α and MAPK Signaling Inhibition, Promoting Antiproliferative Effect and Apoptosis in Glioblastoma Cells

Figure 6

Clonogenic assay and ERK 1/2 protein levels after IP-Se-06 exposure on glioblastoma cells. (a) Crystal violet-stained cultures, , (b) number of colonies formed, (c) colonies discolored with acetic acid 10% and the absorbance measured at 590 nm after treatment with IP-Se-06 (1 μM) at 72 h and/or MEK/ERK inhibitor (U0126) at 10 μM for 1 h. (d) Western blotting of ERK 1/2 in A172 cells treated with IP-Se-06 (1 μM) at 48 h and MEK/ERK inhibitor (U0126) at 10 μM for 1 h. β-Actin was used as loading control. (e) Densitometric analysis result of ERK 1/2 was quantified with ImageJ. The data were considered as statistically significant at compared to the control group (nontreated cells). ^# and ^### indicate a significant difference compared to the treatments.

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