Dietary Fatty Acid Regulation of the NLRP3 Inflammasome via the TLR4/NF-κB Signaling Pathway Affects Chondrocyte Pyroptosis

Figure 7

TLR4 inhibition attenuated SFA- and n-6 PUFA-mediated activation of NLRP3/caspase-1/GSDMD signaling. The relative protein expression of collagen II, TLR4, p-NF-κB, and IL-1β in SW1353 cells treated with PA (a) or LA (b). The relative protein expression of NLRP3, caspase-1, and GSDMD in SW1353 cells treated with PA (c) or LA (d). Immunofluorescent staining was used to detect the expression of caspase-1 (e, g) and GSDMD (f, h) in SW1353 cells treated with PA or LA (200x,  μm). The data are presented as the , . and versus the CON group; ^# and ^## versus the groups without CLI-095-pretreatment; ^ and ^^ versus the LPS group; ^& and ^&& versus the PA or LA group.

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