Dietary Fatty Acid Regulation of the NLRP3 Inflammasome via the TLR4/NF-κB Signaling Pathway Affects Chondrocyte Pyroptosis

Figure 8

TLR4 overexpression reversed the inhibitory effect of n-3 PUFA and MUFA on NLRP3/caspase-1/GSDMD signaling in chondrocytes. The relative protein expression of collagen II, TLR4, p-NF-κB, and IL-1β in SW1353 cells treated with OLA (a) or DHA (b). The relative protein expression of NLRP3, caspase-1, and GSDMD in SW1353 cells treated with OLA (c) or DHA (d). Immunofluorescent staining was used to detect the expression of caspase-1 (e, g) and GSDMD (f, h) in SW1353 cells treated with OLA or DHA (200x,  μm). The data are presented as the , . and versus the NC group; ^# and ^## versus the groups without OE-TLR4-pretreatment; ^ and ^^ versus the NC+LPS group; ^& and ^&& versus the NC+OLA or NC+DHA group.

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