Activation of TGR5 Ameliorates Streptozotocin-Induced Cognitive Impairment by Modulating Apoptosis, Neurogenesis, and Neuronal Firing

Figure 3

INT-777 alleviates STZ-induced decreases of TGR5 expression in the hippocampus and frontal cortex. (a) The level of TGR5 mRNA in the hippocampus and frontal cortex was detected by qRT-PCR. GAPDH was used as a loading control. (b) TGR5 expression of the hippocampus and frontal cortex was detected by western blot. β-Actin was used as a loading control. (c) Quantification of TGR5 level was expressed as the ratio (in percentage) of the Veh+Veh group. (d, e) TGR5-positive cells of the hippocampal CA3 were detected and quantified by immunofluorescence. (f, g) TGR5-positive cells of the mPFC were detected and quantified. Values shown are expressed as the ; mice/group in qRT-PCR and western blot; mice/group, four sections per mouse in immunofluorescence. Scale bar: 100 μm (d, f). vs. the hippocampus or CA3 of STZ+Veh group; ^# and ^## vs. the frontal cortex or mPFC of STZ+Veh group.

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