Sodium Butyrate Ameliorates Oxidative Stress-Induced Intestinal Epithelium Barrier Injury and Mitochondrial Damage through AMPK-Mitophagy Pathway

Figure 5

Effects of sodium butyrate (NaB) on mitophagy of porcine intestinal epithelial cells (IPEC-J2) treated with H2O2. (a, b) mRNA expression level of autophagy-related genes. (c, d) Western blot of autophagy-related protein expression and quantification. (e) The protein expression and quantification of light-chain-associated protein B (LC3B) under a laser confocal microscope. (f) Colocalization of mitochondrial Mito Red and autophagy protein LC3B under a laser confocal microscope. (g) Colocalization of mitophagy proteins PINK1 and LC3B under a laser confocal microscope. (h) Colocalization of mitophagy proteins Parkin and LC3B under a laser confocal microscope. (i) Mitophagy vesicles under transmission electron microscope. Different superscript letters within the same row mean significant difference ().

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