The Ameliorative Role of Eugenol against Silver Nanoparticles-Induced Hepatotoxicity in Male Wistar Rats

Figure 9

Centrilobular zones of hepatic tissues stained with H&E revealing (a) well-organized hepatic strands (HS) which expanded around a narrowed central vein (CV) and segregated by normal blood sinusoids (BS) appeared enveloped by attached endothelial (EC) and Kupffer (KC) cells in control rats; (b) intact hepatic architecture in Eug-treated rats; (c) dilated and congested central vein (CV) possesses sever stagnant hemorrhagic blood (SHB) in its lumen, and its surrounded endothelium seemed destructed (yellow arrow), and the hepatic blood sinusoids (BS) are dilated and congested with stagnant blood (green asterisk), and their Kupffer cells (KC) appeared more rounded and often pushed into the sinusoidal lumen, as well as hepatocytes showed pyknotic nuclei (Pk), and the others revealed nuclear karyorrhexis (Kh) in AgNPs-low-dose-treated rats; (d) severely dilated and congested central vein (CV) having stagnant blood masses (SHB) and infiltrating inflammatory cells (IC) in its lumen and also aggregated in the surrounding connective tissues, besides enlarged blood sinusoids (BS) with swollen and detached Kupffer cells (KC), disorderly hepatic strands with necrotic hepatocytes having pyknotic nuclei (Pk) and rather vacuolated cytoplasm (V) in AgNPs-high-dose-treated rats; (e) conspicuous amelioration in the structure of hepatic strands (HS), blood sinusoids (BS), and central veins (CV) in Eug + AgNPs-low-dose-treated rats; (f) almost intact architecture of the central vein (CV), blood sinusoids (BS), and hepatocytes with some of them still showing rather nuclear pyknosis (Pk) in Eug + AgNPs-high-dose-treated rats.

(a)

(b)

(c)

(d)

(e)

(f)