Research Article
Neurofilament Light Chain (NF-L) Stimulates Lipid Peroxidation to Neuronal Membrane through Microglia-Derived Ferritin Heavy Chain (FTH) Secretion
Figure 4
Influence of FTH-containing exosome on neuronal viability and lipid peroxidation in the presence and absence of FTH1 mediated by siRNA. FTH-containing exosomes were extracted from culture supernatant of BV-2 microglia with 3 μg/ml NFL intervention for 48 h. Then, cortical neuron was treated with the secretory FTH for 24 h in the presence and absence of FTH1 mediated by siRNA. Relative quantification of cell viability (a) and area percentage of C11-Bodipy581/591 (b), respectively; the representative images of C11-Bodipy581/591 (c). Values represent (). and ; ## as compared with the 0 μg/ml NF-L treatment group; ▲▲ as compared with the 3 μg/ml NF-L treatment group in the absence of FTH1.
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