Eugenol-Preconditioned Mesenchymal Stem Cell-Derived Extracellular Vesicles Promote Antioxidant Capacity of Tendon Stem Cells In Vitro and In Vivo

Figure 1

Characterization of BMSCs and EVs. (a) FACS analysis for detection of BMSC surface markers. (b) The osteogenic, chondrogenic, and adipogenic differentiation potentials of BMSCs. Scale bar: 200 μm. (c) Morphology of BMSCs. Scale bar: 250 μm. (d, e) Morphology using transmission electron microscopy. (f) Western blot was used to detect the markers of EVs. (g, h) NTA was used to examine the particle size distribution. (i, j) Internalization of EVs by TSCs. EVs were labeled with DiI PKH26 while the nuclei of TSCs were labeled with Hoechst 33342. Scale bar: 20 μm.

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