Eugenol-Preconditioned Mesenchymal Stem Cell-Derived Extracellular Vesicles Promote Antioxidant Capacity of Tendon Stem Cells In Vitro and In Vivo

Figure 6

EUG-BMSC-EVs protected TSCs against H₂O₂ via Nrf2/HO-1 signaling pathway. (a–c) Western blot was used to examine the expression of NRF2 and HO-1 in H₂O₂-treated TSCs. (d, e) The expression of Nfe2l2 and HO-1 genes. (f) CCK8 assay was performed to assess TSC viability after ML385 treatment. (g, h) Apoptosis of the TSCs after ML385 treatment was detected by flow cytometry. (i, j) The intracellular ROS accumulation was detected by immunofluorescence assay after ML385 treatment. (k, l) Western blot was used to examine the expression of PCNA, catalase, SOD, PARP1, SCXA, TNMD, TNC, COLI, NRF2, and HO-1 after ML385 treatment. (m) The expression of PCNA, Cat, Sod1, SCX, TNMD, TNC, Col1a1, Nfe2l2, and HO-1 genes after ML385 treatment. Bars: 500 μm. Data are represented as . , , and .

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