TREM-1 Modulates Dendritic Cells Maturation and Dendritic Cell-Mediated T-Cell Activation Induced by ox-LDL

Figure 4

TREM-1 regulated ox-LDL-induced DCs inflammation. (a–f) At day 6, DCs were transfected with or without TREM-1 silencer (SiTREM-1) or relevant scrambled siRNA or treated with LP17, and ox-LDL was added 24 h after transfection and further incubated for 24 h. DC supernatants were collected, and cytokine production were tested by ELISA. (g, h) At day 6, DCs were treated with ox-LDL at different concentrations (25, 50, or 100 μg mL⁻¹) for 24 h, IL-12 was determined by ELISA, and 100 ng/mL LPS treatment is a positive control. Results represent the mean ± SD of seven-eight experiments. , compared with control cells; , compared with ox-LDL-treated cells.

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