Research Article
TREM-1 Modulates Dendritic Cells Maturation and Dendritic Cell-Mediated T-Cell Activation Induced by ox-LDL
Figure 6
TREM-1 mediated the Th1/Th17-priming induced by ox-LDL-treated DCs. (a–e) IFN- γ, IL- 17, IL- 4, TGF-β, and IL- 10 content were determined by ELISA. (f) T [DC (siR-TREM-1 + ox-LDL)] cells suppressed primary T-cell proliferation. Naïve CD4+T cells were cultured with ox-LDL- or SiTREM-1 + ox-LDL-treated autologous DCs for 13 days. Naïve CD4+T cells labeled with 5 μmol L-1 CFSES were stimulated with mature DCs (mDCs) alone or in the presence of T [DC], T [DC (ox-LDL)], T [DC (Si-TREM-1 + ox-LDL)], or T [DC (Mock + ox-LDL)] cells at a 1 : 1 ratio. T-cell proliferation was measured by CFSE dilution. Results represent the of seven experiments. , compared with control cells; , compared with ox-LDL-treated cells.
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