Cardiac Fibroblasts Promote Ferroptosis in Atrial Fibrillation by Secreting Exo-miR-23a-3p Targeting SLC7A11

Figure 3

GW4869 attenuates the degradation of ferroptosis-associated proteins and the secretion of exosomes in atrial tissue of rapid atrial pacing in canines. (a) Representative gel bands depicting exosome markers and ferroptosis-associated proteins expression using specific antibodies. GAPDH was used as the loading control. (b) Levels of CD63, CD81, and TSG101. ( =5). (c) Levels of FTH1, GPX4, and SCL7A11. ( =5). (d, f) Representative images of inflammatory cell infiltration, ferroptosis, fibrosis, and SLC7A11 as reflected by H&E staining, Prussian staining, Masson staining, and immunohistochemistry. ( =3). (e, g) Representative images of immunofluorescence staining for FTH1 protein in canines atrial tissue stimulated by rapid pacing with or without GW4869. ( =3). (h) RT-PCR analysis of FTH1, GPX4, and SLC7A11 expression normalized with GAPDH. ( =5). Data are presented as the mean ± SD. Statistical significance was determined using one-way ANOVA with a post hoc Dunnett test. , , and vs. Sham group; , , and vs. Pacing group.

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