Biogenic Selenium Nanoparticles Alleviate Intestinal Epithelial Barrier Damage through Regulating Endoplasmic Reticulum Stress-Mediated Mitophagy

Figure 1

Effects of SeNPs on ER structure and function of IPECJ2 cells exposed to H₂O₂. (a) Protective effect of SeNPs on the ultrastructure of ER and mitochondria in IPEC-J2 cells exposed to H₂O₂. (b) Regulatory effect of SeNPs on the mRNA levels of ER-resident selenoproteins. (c) Intracellular Ca²⁺ concentration was detected by Fluo-4 AM staining (scale bar: 100 μM). (d) Quantitative analysis of intracellular Ca²⁺ concentration. (e) Intracellular IP3 content. (f) The expression level of IP3R was detected by Western blot analysis. (g) Quantified statistical result of IP3R expression level. All data are presented as the of three independent experiments. , , and . SeNPs: selenium nanoparticles; ER: endoplasmic reticulum; H₂O₂: hydrogen peroxide; IP3: inositol triphosphate; IP3R: inositol trisphosphate receptor.

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