Research Article
Biogenic Selenium Nanoparticles Alleviate Intestinal Epithelial Barrier Damage through Regulating Endoplasmic Reticulum Stress-Mediated Mitophagy
Figure 10
Effect of AMPK agonist and inhibitor on ERS-mediated mitophagy in IPEC-J2 cells exposed to H2O2. (a) The expression levels of AMPK and p-AMPK were detected by Western blot analysis. (b) The expression levels of Beclin1 and SQSTM1 were detected by Western blot analysis. (c) The expression level of LC3B-II/LC3B-I was detected by Western blot analysis. (d) The ROS levels were detected by DCFH-DA fluorescent probe (scale bar: 50 μM). (e) Quantitative analysis of intracellular ROS level. (f) The expression levels of occludin and claudin-1 were detected by Western blot analysis. All data are presented as the of three independent experiments. , , and . SeNPs: selenium nanoparticles; H2O2: hydrogen; SQSYM1: sequestosome-1; LC3B: microtubule-associated protein light chain 3; ROS: reactive oxygen species.
| (a) |
| (b) |
| (c) |
| (d) |
| (e) |
| (f) |