Biogenic Selenium Nanoparticles Alleviate Intestinal Epithelial Barrier Damage through Regulating Endoplasmic Reticulum Stress-Mediated Mitophagy

Figure 9

Effect of PERK agonist and inhibitor on ERS-mediated mitophagy in IPEC-J2 cells exposed to H₂O₂. (a) The expression levels of PERK signaling pathway-related proteins were detected by Western blot analysis. (b) The expression levels of AMPK, p-AMPK, mTOR, and p-mTOR were detected by Western blot analysis. (c) The expression levels of Beclin1 and SQSTM1 were detected by Western blot analysis. (d) The expression level of LC3B-II/LC3B-I was detected by Western blot analysis. (e) The ROS levels were detected by DCFH-DA fluorescent probe (scale bar: 50 μM). (f) The expression levels of occludin and claudin-1 were detected by Western blot analysis. All data are presented as the of three independent experiments. , , and . SeNPs: selenium nanoparticles; H₂O₂: hydrogen; PERK: protein kinase R-like endoplasmic reticulum kinase; eIF2α: eukaryotic initiation factor 2; AMPK: AMP-activated kinase; mTOR: mechanistic target of rapamycin; SQSYM1: sequestosome-1; LC3B: microtubule-associated protein light chain 3.

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