Research Article
Oxidative Stress Aggravates Apoptosis of Nucleus Pulposus Cells through m6A Modification of MAT2A Pre-mRNA by METTL16
Figure 4
The effect of METTL16 on MAT2A. (a) In degenerative human NP tissues, qRT-PCR showed that exon1-3 and harpin1 reflecting the total of MAT2A pre-mRNA and MAT2A mRNA decreased, while intron8 reflecting MAT2A pre-mRNA increased. (b) In the NPCs stimulated by TBHP, qRT-PCR showed exon1-3 and harpin1 decreased while intron8 increased. (c) In the NPCs with METTL16 overexpression, qRT-PCR showed MAT2A exon1-3 and harpin1 decreased, while intron8 increased. (d) Immunofluorescence showed that the expression of MAT2A protein decreased when METTL16 was upregulated in NPCs. The expression of MAT2A was higher in the NPCs with unsuccessful overexpression of METTL16 (white arrow) than in the cells with successful overexpression of METTL16 (black arrow). (e) MAT2A and METTL16 proteins detected by Western blot in cells with METTL16 overexpression. replicates per group, , , .
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