Inhibition of TRPA1 Ameliorates Periodontitis by Reducing Periodontal Ligament Cell Oxidative Stress and Apoptosis via PERK/eIF2α/ATF-4/CHOP Signal Pathway

Figure 1

Periodontitis-derived periodontal ligament cells and tissues were at high levels of oxidative stress and apoptosis. (a) The related gene expressions of oxidative stress, apoptosis, and some of the TRP families in healthy and periodontitis-derived periodontal ligament cells (PDLCs and P-PDLCs) (). (b, c) Western blot and semiquantitative statistical analysis of oxidative stress, apoptosis, and TRPA1 in PDLCs and P-PDLCs. (). d, Flow cytometry analysis of PDLCs and P-PDLCs (). (e, f) H&E staining (white star represent immune cell infiltration), immunohistochemistry and immunofluorescence staining, and semiquantitative statistical analysis of periodontitis and healthy derived periodontal ligament tissues (PDLTs and P-PDLTs) (). Data analysis was performed by using Student’s -test (, , and ). The data are presented as the .

(a)

(b)

(c)

(d)

(e)

(f)