Laser Capture Microdissection in the Spatial Analysis of Epigenetic Modifications in Skin: A Comprehensive Review

Table 1

LCM-based studies for cutaneous manifestations.

Study and author	Disease model	Tissue element captured	Type of epigenetic modification studied
Singh et al. [42]	Diabetes	CD31^+ endothelial element	Methylation of miR-200b promoter
Ren et al. [8]	Ovarian cancer	Tissue from ovarian carcinoma	Inactivation of hMLH1 during malignancy
Li et al. [9]	Aging	Dermal and epidermal elements	Expression of PTGES1 and COX2 mRNA expression
Wu et al. [36]	Endometriosis	Epithelial component of endometriotic implants	Methylation status of progesterone receptors (PRA, PRB)
Greenspan et al. [2]	Colon tumorigenesis	Colonic epithelial elements	Methylation status of RASSF1A
Sigalotti et al. [39]	Melanoma	Cutaneous melanoma cellular elements	Methylation of LINE-1
Nie et al. [41]	Chronic wounds	Diabetic ulcers	miRNA expression of NFkB, TGF-B/SMED
Singh et al. [43]	Wound angiogenesis	Human dermal microvascular endothelial cells	E-Cadherin/ZEB1 expression
Wu et al. [53]	Cutaneous T cell lymphoma	Human CTCL lines HH, Sz4, MyLa, Hut 78	CpG methylation of FAS/CD95
Masterson et al. [54]	Merkel cell carcinoma	Tumor resection epithelial elements	Expression of KRT20, KIF3A, and MUCI1