Inhibiting Microglia-Derived NLRP3 Alleviates Subependymal Edema and Cognitive Dysfunction in Posthemorrhagic Hydrocephalus after Intracerebral Hemorrhage via AMPK/Beclin-1 Pathway

Figure 6

IL-1beta upregulated neurons autophagy and apoptosis, and inhibiting autophagy could reduce neurons apoptosis mediated by IL-beta which was released after NLRP3 activated. (a) Representative immunofluorescence staining images of Atg5- and p62-positive PC12 cells. . (b) Immunofluorescence TUNEL-staining images of PC12 cells, and statistic result of TUNEL(+) cells after different treatments. . (c) Representative Western blot images of LC3B and p62 expressions in PC12 cells, and statistic results of LC3B-II : LC3B-I ratio and p62 after IL-1beta and compound C treated. (d) Representative images and statistic results of flow cytometry showed early/late apoptotic cells after IL-1beta and compound C treated. versus sham group, ^## IL-1beta group versus IL-1beta + compound C group.

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