4,7-Didehydro-neophysalin B Protects Rat Lung Epithelial Cells against Hydrogen Peroxide-Induced Oxidative Damage through Nrf2-Mediated Signaling Pathway

Table 2

Primers used in RT-PCR.

Gene	Primer (5→3)
Nrf2	Forward: GAGAGCCCAGTCTTCATTGC; Reverse: TTGGCTTCTGGACTTGGAAC
Keap1	Forward: TTCAAGGCCATGTTCACCAA
	Reverse: TGGATACCCTCAATGGACACC
NQO1	Forward: GGAGAGTTTGCTTACACTTACGC
	Reverse: AGTGGTGATGGAAAGCACTGCCTTC
HO-1	Forward: CGCCTTCCTGCTCAACATT
	Reverse: TGTGTTCCTCTGTCAGCATCAC
KLF9	Forward: GGGAAACCTCCGAAAA
	Reverse: CGTTCACCTGTATGCACTGTA
GAPDH	Forward: GGAGATTACTGCCCTGGCTC; Reverse: GACTCATCGTACTCCT

RT-PCR was performed on the ABI PRISM® 7500 real-time PCR analyzer (Applied Biosystems, Foster City, CA, USA) using the SYBR® Premix Ex Taq™ RT-PCR Kit. The relative mRNA expression was calculated by means of 2^−ΔΔCt and was normalized to the mean mRNA expressions of GAPDH. The results were calculated with the following formulae: , .