Inhibition of PLK3 Attenuates Tubular Epithelial Cell Apoptosis after Renal Ischemia–Reperfusion Injury by Blocking the ATM/P53-Mediated DNA Damage Response

Figure 4

Targeted silencing of PLK3 expression reduces H/R-induced apoptosis in TECs. (a) qRT–PCR analysis of PLK3 mRNA expression after transfection with 100 nM PLK3-siRNA or scrambled oligonucleotide (si-NC). (b) Immunoblotting analysis of PLK3 silencing efficiency. (c) The viability of HK-2 cells was measured by CCK-8 assays. (d) Flow cytometry was performed to examine HK-2 cell apoptosis. (e) Representative images showing TUNEL-positive HK-2 cells after PLK3 silencing (; magnification, 400x). The data are presented as the . . , , , and .

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