Chitosan Oligosaccharides Alleviate H₂O₂-stimulated Granulosa Cell Damage via HIF-1α Signaling Pathway

Figure 6

The effect of the COS treatment on the HIF-1α/VEGF pathway in H₂O₂-stimulated KGN cells. (a) The protein expressions of HIF-1α and VEGF. (b) The statistics histogram of western blotting is expressed as band density normalized versus actin. (c) The mRNA expressions of HIF-1α and VEGF. (d) Determination of senescence in KGN cells by staining for SA-β-gal (200X). (e) Statistical analysis of quantification of the SA-β-gal-positive cells. (f–i) The levels of IL-6, IL-10, E₂, and P₄. Data are presented as the (). , , or vs. the control group; ^#, ^##, or ^### vs. the H₂O₂ group; ^&, ^&&, or ^&&& vs. the H₂O₂+100 COS+KC7F2 group.

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