Research Article
Chitosan Oligosaccharides Alleviate H2O2-stimulated Granulosa Cell Damage via HIF-1α Signaling Pathway
Figure 8
The effect of the COS treatment on the HIF-1α/VEGF pathway in H2O2-stimulated KGN cells. (a) Production of ROS determined by a fluorescent probe. (b) MitoTracker was stained on KGN cells by immunofluorescence. (c) JC-1 red, JC-1 green, and merge image. (d, e) The statistics histogram of immunofluorescence expressed fluorescent intensity. (f) Statistics data were expressed in terms of the ratio of JC-1 red to JC-1 green. Data are presented as the (). , , or vs. the control group; #, ##, or ### vs. the H2O2 group; &, &&, or &&& vs. the H2O2+100 COS+KC7F2 group. Scale bar: (a) ROS/MitoTracker: 100 μm; JC-1 red/JC-1 green/merge: 50 μm.
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