Coenzyme Q₀ Inhibits NLRP3 Inflammasome Activation through Mitophagy Induction in LPS/ATP-Stimulated Macrophages

Figure 4

CoQ₀ induces mitophagy in LPS/ATP-stimulated RAW264.7 macrophages. (a) Time-dependent expression of p-PI3K, PI3K, p-AKT, AKT, p-p70 S6 kinase, p70 S6 kinase, p-mTOR, and mTOR was determined by Western blot. Cells were first treated with CoQ₀ (10 μM) for 0-60 min and then stimulated with LPS (1 μg/mL) for 5 h followed by ATP (5 mM) for 1 h. (b) Dose-dependent expression of p-p70 S6 kinase and p-AKT was determined by Western blot. Cells were pretreated with CoQ₀ (2.5-10 μM) for 60 min and then LPS (1 μg/mL) stimulation for 5 h followed by ATP (5 mM) for 1 h, and the changes in the intensities of protein bands were measured by commercial quantitative software. (c) The cells were first treated with CoQ₀ (2.5-10 μM) for 60 min and then stimulated with LPS (1 μg/mL) for 5 h followed by ATP (5 mM) for 1 h, and lastly, expression of Parkin and PINK1 was determined by Western blot.

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