Investigating the Mechanisms of Jieduquyuziyin Prescription Improves Lupus Nephritis and Fibrosis via FXR in MRL/lpr Mice

Figure 8

ELISA detected (a, b) the contents of IL-6 and TNF-α in the supernatant of HK-2 cells transfected and treated with LPS (1 μg/mL, 6 h). Data are expressed as the deviation (), versus the blank group; , versus the control group. (c–e) WB detection of transfected FXR overexpression or interfering RNA and protein expression of HK-2 cells after stimulation with 10% concentration drug-containing serum and treatment with TGF-β (20 ng/mL, 24 h). Data are expressed as the deviation (), , . WB result (f–h) observation of protein expression after transfection of siFXR or siα-SMA and treatment with TGF-β (20 ng/mL, 24 h). versus the control group; ns: not statistically significant.

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