Lipopolysaccharide Enhances Genotoxicity by Activating GADD45G and NF-κB in Human Corneal Epithelial Cells

Figure 6

An analysis mRNA expression profiles of DNA damage, growth arrest, and DNA repair genes in HCECs treated with lipopolysaccharide (LPS). The following qRT-PCR experiments were carried out using LPS treated HCEC cells to examine mRNA levels for GADD45G, PARP1, Tp53, MSH2, OGG1, BRCA1, DDB1, and XRCC1 (a)–(h). Quantification of the mRNA expression was performed at time points of 0, 1, 2, 4, 8, 12, and 24 hours. Based on three cultures replicated for each experimental points (), the data represent (S.E.). values of 0.05, 0.01, and 0.001 representing , , and as compared to untreated control cells (NC) were calculated using Kruskal-Wallis and Dunn’s tests.

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