Lipopolysaccharide Enhances Genotoxicity by Activating GADD45G and NF-κB in Human Corneal Epithelial Cells

Figure 8

In lipopolysaccharide- (LPS-) treated HCECs, survival growth factor, growth arrest, and DNA damage-inducible protein expression have been examined. Western blots showing photographic images of cell Akt and Erk (a). An immunoblot showing the Gadd45γ, pNF-κB (p65), and phosphor γH2AX protein expression (b). In (c), the graph shows the levels of Akt, p-Akt, Erk, and p-Erk protein expression, which were determined by densitometry analysis using the Image-J software. The graph (d)–(f) show the expressions of the Gadd45γ, pNF-κB (p65), and phosphor γH2AX. These expressions are quantified by densitometric analysis using Image-J software. The relative density bands of the target gene were normalized to expression of the housekeeping gene β-actin. Based on three cultures replicated for each experimental points (), the data represent (S.E.). values of 0.05, 0.01, and 0.001 representing , , and as compared to untreated control cells (NC) were calculated using Kruskal-Wallis and Dunn’s tests.

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