Identification of Resolvin D1 and Protectin D1 as Potential Therapeutic Agents for Treating Kidney Stones

Figure 2

RvD1 and PD1 inhibited CaOx deposition-induced cell damage. A mouse model of CaOx deposition was constructed, and the effects of RvD1 and PD1 on CaOx deposition were observed after the administration of different concentrations of RvD1 (18.75 and 31.25 μg/kg) and PD1 (11.25 and 18.75 μg/kg). (a) Representative map of CaOx deposition in the whole kidney as observed by polarized light optical microscopy (×20, scale bar: 500 μm). Pizzolato staining was performed to examine CaOx deposition in local tissues (×200; scale bar: 20 μm). PAS staining was performed to examine cell damage (×200; scale bar: 20 μm). A TUNEL assay was performed to examine apoptosis (×200; scale bar: 100 μm). (b) The ratio of the areas of kidneys with crystal deposition, as determined by polarized light optical microscopy. (c) The ratio of the areas of kidneys with corticomedullary junction area crystal deposition. (d) The tubular injury score was determined by PAS staining. (e) The average number of TUNEL-positive cells per high-power field. (f, g) BUN and serum creatinine were used to assess renal function (). The data are the of three independent experiments. One representative plot of mice is shown. #; ## compared with NC, ; compared with Gly, one-way ANOVA (b–g).

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