Deep Learning-Assisted Repurposing of Plant Compounds for Treating Vascular Calcification: An In Silico Study with Experimental Validation

Figure 6

Validating the positively predicted candidates (a) sulforaphane, (b) daidzein, and (c) catechin, a candidate with a neutral effect in an in vitro vascular calcification model. Calcification nodules were stained using Alizarin Red staining and imaged without (upper row) and with different magnification levels (mid and lower rows) under microscopic examinations. Comparisons of calcium deposition quantities were done between groups using Student’s -tests. , , . C: catechin; Ctrl: control; Daid: daidzein; HP: high phosphate; Sulf: sulforaphane.

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