Effect of Aronia Extract on Collagen Synthesis in Human Skin Cell and Dermal Equivalent

Figure 2

Aronia extracts promote collagen synthesis in human dermal fibroblasts. (a) Expression analysis of COL1A1 in HDFn cells. The HDFn cells were treated with the indicated concentration of Aronia extract for 48 h. Then, relative COL1A1 mRNA levels were measured by qRT-PCR using the indicated gene-specific primers. Bars represent the (). Control (Cont). (b) Aronia extract elevated COL1A1 promoter activity. Luciferase reporter assays were performed in HDFn cells (). (c) Schematic representation of reporter gene constructs. Reporter construct composed of the psiCHECK2-backbone plasmid containing the Renilla luciferase gene and human COL1A1 3UTR (RL-COL1A1-3U). (d) COL1A1 mRNA levels in HDFn cells treated with actinomycin D (Act.D) for 2, 4, 6, and 8 h. -axis shows the relative levels of COL1A1 mRNA ().

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