Effect of Aronia Extract on Collagen Synthesis in Human Skin Cell and Dermal Equivalent

Figure 3

Aronia extracts downregulate MMPs. (a and b) MMP1(a) and MMP3(b) expression levels in HDFn cells. The HDFn cells were treated with the indicated concentration of Aronia extract for 24 h, and MMP1 and MMP3 mRNA levels were measured by qRT-PCR using the indicated gene-specific primers. The value of the vehicle was 1 (). (c) Immunoblots for COL1A1 and MMP-1 in HDFn cells treated with Aronia extracts. β-Actin (ACTB) was a loading control. Aronia extracts increased COL1A1 protein levels and decreased MMP-1 protein levels in HDFn cells.

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