(–)-Epicatechin Alters Reactive Oxygen and Nitrogen Species Production Independent of Mitochondrial Respiration in Human Vascular Endothelial Cells

<div>EPI rapidly and transiently activates ERK1/2 signalling whilst blunting AMPK phosphorylation. (a) AMPK<svg height="6.1673pt" id="M94" style="vertical-align:-0.2063904pt" version="1.1" viewbox="-0.0498162 -5.96091 7.51131 6.1673" width="7.51131pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M545 106L524 126C493 85 467 65 455 65C438 65 427 113 405 238C448 295 498 362 543 439L533 448L478 435C453 386 423 331 398 295H395C370 404 347 448 282 448C169 448 23 309 23 153C23 54 65 -12 128 -12C203 -12 283 70 339 155H341C360 29 380 -12 411 -12C444 -12 491 11 545 106ZM333 204C265 95 210 54 169 54C137 54 113 96 113 171C113 302 191 405 252 405C301 405 318 306 333 204Z"></path></g></svg> phosphorylation at Thr172 in HUVECs in the absence (-; clear bars) or presence (+; green bars) of EPI. (b) ERK1/2 phosphorylation at Thr202/Tyr204. (c) eNOS phosphorylation at Ser1177. (d) Representative images of <svg height="8.55521pt" id="M95" style="vertical-align:-0.2063904pt" version="1.1" viewbox="-0.0498162 -8.34882 27.8368 8.55521" width="27.8368pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M495 86L479 114C446 82 419 66 409 66C401 66 401 72 406 97C420 166 436 231 453 297C489 435 454 448 428 448C406 448 384 439 354 422C305 394 222 327 161 247H159L183 345C200 415 194 448 173 448C143 448 82 410 23 351L38 325C64 349 95 371 105 371C111 371 116 365 109 336L25 -4L31 -12C50 -4 77 3 107 9C119 69 132 122 145 168C197 254 321 381 370 381C387 381 393 374 378 305L329 95C309 17 320 -12 345 -12C372 -12 430 19 495 86Z"></path></g><g transform="matrix(.013,0,0,-0.013,10.158,0)"><path d="M535 323V373H52V323H535ZM535 138V188H52V138H535Z"></path></g><g transform="matrix(.013,0,0,-0.013,21.421,0)"><path d="M285 378C315 398 338 416 353 432C373 451 384 474 384 503C384 579 325 635 236 635H235C182 635 136 610 108 579L65 516L85 496C110 533 150 575 205 575C258 575 300 543 300 481C300 407 232 369 141 339L147 310C163 315 188 321 211 321C268 321 338 284 338 192C338 94 288 40 217 40C160 40 119 68 93 91C85 98 77 97 69 91C60 84 47 71 46 58C44 46 48 35 62 22C75 10 116 -12 162 -12C234 -12 424 62 424 224C424 297 373 359 285 376V378Z"></path></g></svg> independent experiments. Cell lysates were analysed by SDS-PAGE and western blotting with indicated antibodies. Data are expressed as <span class="nowrap"><svg height="9.10327pt" id="M96" style="vertical-align:-0.3499298pt" version="1.1" viewbox="-0.0498162 -8.75334 72.3287 9.10327" width="72.3287pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M797 0V26C739 32 732 36 732 103V296C732 394 682 449 605 449C576 449 550 437 529 423C504 407 475 389 446 366C425 418 382 449 334 449C303 449 279 437 253 421C222 403 201 385 180 371V452C135 432 85 419 41 411V388C99 379 102 374 102 310V103C102 38 93 32 27 26V0H238V26C189 32 180 38 180 103V338C210 363 250 390 289 390C351 390 377 348 377 275V103C377 37 368 32 306 26V0H520V26C465 32 456 38 456 101V296C456 314 455 326 453 338C491 369 529 390 565 390C628 390 653 345 653 274V107C653 36 642 32 583 26V0H797Z"></path></g><g transform="matrix(.013,0,0,-0.013,10.595,0)"><path d="M380 106C343 72 306 56 265 56C195 56 116 112 115 248C235 252 361 262 377 265C396 269 400 277 400 297C400 374 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338 206Z"></path></g></svg>Significant main effect of treatment; <svg height="12.308pt" id="M100" style="vertical-align:-0.04979992pt" version="1.1" viewbox="-0.0498162 -12.2582 5.95946 12.308" width="5.95946pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.0091,0,0,-0.0091,0,-5.741)"><path d="M563 583C563 664 501 710 433 710C381 710 334 692 285 648C212 582 178 506 149 350L74 -56C49 -190 36 -222 24 -226L30 -257C52 -256 95 -247 121 -229C131 -222 134 -206 165 -21L219 303C254 510 296 665 397 665C447 665 481 626 481 572C481 512 446 457 390 420C367 405 351 400 323 397L302 349C398 338 466 285 466 197C466 117 419 45 306 45C268 45 224 59 198 74L186 50C197 20 228 -14 274 -14C318 -14 359 3 401 26C478 68 554 144 554 225C554 322 493 367 412 396C490 446 563 500 563 583Z"></path></g></svg>significant main effect of time (<span class="nowrap"><svg height="9.2729pt" id="M101" style="vertical-align:-0.6370001pt" version="1.1" viewbox="-0.0498162 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Oxidative Medicine and Cellular Longevity

fig5

Figure 5

Figure 5: (–)-Epicatechin Alters Reactive Oxygen and Nitrogen Species Production Independent of Mitochondrial Respiration in Human Vascular Endothelial Cells 

Figure 5 | (–)-Epicatechin Alters Reactive Oxygen and Nitrogen Species Production Independent of Mitochondrial Respiration in Human Vascular Endothelial Cells 