Dopamine D2 Receptor Signaling Attenuates Acinar Cell Necroptosis in Acute Pancreatitis through the Cathepsin B/TFAM/ROS Pathway

Figure 1

DRD2 activation with quinpirole reduced acinar cell necroptosis during AP in vivo and in vitro. In vivo AP was induced by caerulein and LPS or NaT. DRD2 agonist quinpirole was administered intraperitoneally 0.5 h before the injection of caerulein or NaT. In vitro PACs were pretreated with quinpirole (5 μM) for 0.5 h and then stimulated with 200 nM CCK for 6h. (a) Representative graphs of H&E-stained pancreatic tissue (=6~7 per group, scale bar 100 μm). (b) Histological scores from NaT-induced pancreatitis (=6~7 per group). ELISA of (c) amylase and (d) lipase in serum (=6~7 per group). (e, f) Immunoblot analysis and quantification of RIP3, p-MLKL, and MLKL proteins of pancreas in mice treated with NaT ( =5 in each group). (g, h) Immunoblot analysis and quantification of RIP3, p-MLKL, and MLKL proteins of pancreas in mice treated with caerulein and LPS ( =5 in each group). (i, j) Immunoblot analysis and quantification of RIP3, p-MLKL, and MLKL proteins of pancreatic acinar cells with CCK stimulation ( =4 in each group). (k, l) Immunoblot analysis and quantification of CTSB proteins of pancreas in mice ( =3 per group). , , and .

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