Dopamine D2 Receptor Signaling Attenuates Acinar Cell Necroptosis in Acute Pancreatitis through the Cathepsin B/TFAM/ROS Pathway

Figure 7

CTSB bound to TFAM and negatively regulated its expression. (a, b) Isolated PACs were pretreated with CA-074Me (10 μM) for 0.5 h and then stimulated with 200 nM CCK for 6 h. Immunoblot analysis and quantification of TFAM levels of PACs with CCK stimulation ( per group). (c, d) AP was induced by caerulein and LPS. CA-074Me was administered intraperitoneally 0.5 h before the injection of caerulein. Immunoblot analysis and quantification of TFAM levels of pancreas in mice treated with caerulein and LPS (=4 per group). (e) Isolated PACs were treated with LLOMe (0.5 mM) for 6 h. Co-ip experiment results suggested TFAM protein bound to CTSB protein. , , and .

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