Dopamine D2 Receptor Signaling Attenuates Acinar Cell Necroptosis in Acute Pancreatitis through the Cathepsin B/TFAM/ROS Pathway

Figure 8

Knockdown of TFAM reversed the protective effect of either CA-074Me or quinpirole in vitro. PACs were isolated and infected with adenovirus for 6 h. After 18 h, PACs were pretreated with CA-074Me (10 μM) or quinpirole (5 μM) for 0.5 h and then stimulated with 200 nM CCK. (a, b) Immunoblot analysis and quantification of TFAM proteins in PACs after Adv-shTFAM infection compared to Adv-Con infection group. Cell injury determined by (c) the percentage of ATP depletion and (d) LDH leakage (=4 per group). (e, f) Representative images and quantification of DHE-stained PACs showing the levels of superoxide anions (=3 per group, scale bar 100 μm). (g, h) Immunoblot analysis and quantification of RIP3, p-MLKL, and MLKL proteins of PACs (=4 per group). , , and .

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