Quercetin Protects Ethanol-Induced Hepatocyte Pyroptosis via Scavenging Mitochondrial ROS and Promoting PGC-1α-Regulated Mitochondrial Homeostasis in L02 Cells

Figure 1

Hepatoprotective effects of quercetin against ethanol exposure. (a) Effects of different concentrations of ethanol on cell viability treated with different concentrations of ethanol for 24 h. (b) Quantitation of levels of ADH and CYP2E1 in L02 cells treated with different concentrations of ethanol for 24 h. (c, d) Quantitation of levels of ADH, CYP2E1, and ALDH2 treated with different concentrations of ethanol for 24 h. (e) Representative images of L02 cells and microphotograph of Oil-Red O staining (200x magnification). (f) Representative immunofluorescent images costained with Nile Red (red), DAPI (blue), and both channels merged. The graphs (down panel) show the fluorescence intensity profiles in two fluorescence channels along the arrow (200x magnification). (g) Determination of TAS, TOS levels, and . (h) Determination of LPO level. (i) Representative TEM images of mitochondrial morphology and ultrastructure in L02 cells with boxed areas enlarged. M: mitochondria; N: nucleus; lipid drops (orange arrow) in all groups. Magnification is shown (scale bar, 2.0 μm). Data are expressed as (). Different subscript letters indicate significant differences among the groups ().

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)