Quercetin Protects Ethanol-Induced Hepatocyte Pyroptosis via Scavenging Mitochondrial ROS and Promoting PGC-1α-Regulated Mitochondrial Homeostasis in L02 Cells

Figure 2

Quercetin reduced ethanol-induced mitROS generation and maintained mitochondrial dynamics. (a) Images of MitoSox Red staining for mitochondrial superoxide (200x magnification), the graphs (down panel) show the fluorescence intensity profiles in two fluorescence channels along the arrow. (b) Quantitative analysis of fluorescence intensity and mitROS generation by flow cytometry. (c) Representative immunofluorescent images co-stained with Mito-Tracker (green), DAPI (blue), and both channels merged (400× magnification). The graphs (up panel) show the fluorescence intensity profiles in two fluorescence channels along the arrow, and the white arrows represent fission; the yellow arrowheads show fusion. (d, e) Quantitative analysis of mitochondrial fission and fusion ratio and fission length. (f–h) The mRNA expression of mitochondrial function genes (VDAC, COXIV, and PINK1), mitochondrial fission genes (Mff, Drp1, and Fis1) and fusion genes (Opa1, Mfn1, and Mfn2). Data are expressed as (). Different subscript letters indicate significant differences among the groups ().

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