Quercetin Protects Ethanol-Induced Hepatocyte Pyroptosis via Scavenging Mitochondrial ROS and Promoting PGC-1α-Regulated Mitochondrial Homeostasis in L02 Cells

Table 2

The gene primer sequences used for RT-qPCR.

Gene name	Forward primer (5->3)	Reverse primer (5->3)
ADH1	TGGGAAAAGTATCCGTACCATT	AGCTGTTGCTCCAGATCATGT
ADH2	GGGCAGAGAAGACAGAAACGA	TTCCTCTCATCAAACTTCTGTCA
ADH3	CCCAAACTTGTGGCTGACTTT	ACTCTTTCCAGAGCGAAGCA
CYP2E1	GAAGCCTCTCGTTGACCCAA	TGGTGGGATACAGCCAAACC
ALDH2	CATGGACGCATCACACAGGG	CTTGCCATTGTCCAGGGTCT
COXIV	GGCGGCAGGTGTACATTTTTA	AGTCTTCGCTCTTCACAACA
VDAC	CCCAAACTTGTGGCTGACTTT	ACTCTTTCCAGAGCGAAGCA
PINK1	AACCGCTTCGACTTTCTGCT	CACTTGATGAACCAGCCCCA
Mfn1	TGGCCACATGTAGTTTATGTTTC	TTGCACCTGCTGTAAAAAGGC
Mfn2	GGAAGGTGAAGCGCAATGTC	TGCATTCACCTCAGCCATGT
Opa1	CTGTGGCCTGGATAGCAGAA	AGACTGGCAGACCTCACTCT
Drp1	CCGGAGACCTCTCATTCTGC	TCTGCTTCCACCCCATTTTCT
Fis1	TCAGCCCCATCATGTGCTTT	CAGGAGAGGACCAGGAGTGA
Mff	CTCTCAGCCAACCACCTCTG	GTGCTGGATTGAGAGCCACT
TWNK	CTGGTTGGGGGATGCCTTC	ATTGAAGCCTCCGTTCAGGG
MFND	CAACTACGCAAAGGCCCCA	TGATGGTAGATGTGGCGGGT
MTCO1	CTATCCGGAATGCCCCGA	GGCATCCATATAGTCACTCCAG
IL-18	TGCAGTCTACACAGCTTCGG	GCAGCCATCTTTATTCCTGCG
IL-1β	TGATGGCTTATTACAGTGGCA	CGGAGATTCGTAGCTGGATG
Caspase-1	CCTGCCGTGGTGATAATGTT	TCCACATCACAGGAACAGGC
GSDMD	CAGAAGGGACGTGGTGTTCC	AGTTTACGGAAGTCGGCGAG
GAPDH	ACTAGGCGCTCACTGTTCT	CCAATACGACCAAATCCGTTG