Attenuation of ROS/Chloride Efflux-Mediated NLRP3 Inflammasome Activation Contributes to Alleviation of Diabetic Cardiomyopathy in Rats after Sleeve Gastrectomy

Figure 3

Effects of SG on diabetic-induced cardiac remodeling. (a) Representative (a1) longitudinal and (a2) transverse H&E staining of left ventricle. Scale bar, 50 μm. (b) Staining of Masson trichrome; dark blue indicates collagen fibers. Scale bar, 50 μm. (c) Sirus red staining; note the dense collagen staining. Scale bar, 50 μm. Immunohistochemical staining of (d) collagen I and (e) collagen III. Scale bar, 50 μm. (f) Oil Red O staining quantifies lipid accumulation. Scale bar, 50 μm. (g) The TUNEL assay was performed to identify DNA nicks; red plots indicate TUNEL-positive cells. Scale bar, 50 μm. (h) Representative transmission electron micrographs. Scale bar, 2 μm (h) Quantitative analysis of the cardiomyocyte size. (i) Semiquantification of collagen volume in total left ventricle. Data are presented as . CON vs. SHAM; ^## SG vs. SHAM. in each group.