Protective Effects of Cannabidiol on Chemotherapy-Induced Oral Mucositis via the Nrf2/Keap1/ARE Signaling Pathways

Figure 8

CBD plays protective effects on OM via the Nrf2/Keap1/ARE pathway. HOK cells were transfected with Nrf2-siRNA and Scramble-siRNA, respectively. After 6 hrs transfection, HOK cells were treated with 5-FU and CBD. Then, the cells were collected for the following analysis. (a) Knockdown efficiency of Nrf2 as measured by qRT-PCR. vs. vehicle group. (b) Detection of Nrf2, HO-1, and NQO1 protein levels by western blotting. β-Actin was used as the internal control. (c) Grayscale analysis of the blots. (d) The mRNA expression levels of Nrf2, HO-1, and NQO1 as measured by qRT-PCR. β-Actin was used as the internal control. (e) DCFH-DA staining. Scale bar, 200 μm. (f) Quantitative analysis of the mean fluorescence intensity. Data are expressed as the . vs. the 5-FU alone group; ^#vs. the 5-FU+CBD group.

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