Melatonin Alleviates Acute Kidney Injury by Inhibiting NRF2/Slc7a11 Axis-Mediated Ferroptosis

Figure 1

Melatonin failed to alleviate the HR-induced ferroptosis in NRF2-silenced MTEC. (a) Real-time PCR and (b) western blot analysis of the expression of NRF2 in MTEC after transfection with control siRNA or three alternative NRF2 siRNAs (NRF2 siRNAs 1, 2, and 3), respectively. (c) Western blotting showing the expression of Slc7a11 and GPX4 proteins in control-siRNA and NRF2-siRNA cells treated with HR and 1 mM melatonin. (d–f) The levels of SOD, GSH-Px, and MDA in control-siRNA and NRF2-siRNA cells treated with HR and 1 mM melatonin were measured. Data represent the of three to four independent experiments. compared with the NC group. compared with the HR-treated group.

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