Melatonin Alleviates Acute Kidney Injury by Inhibiting NRF2/Slc7a11 Axis-Mediated Ferroptosis

Figure 2

Melatonin treatment alleviates IR-induced acute kidney injury in mice. (a) Serum ALT. (b–d) BUN, serum creatinine (Scr), and Ccr level; the result shows that MT or Fer-1 could alleviate the kidney injury in IR-AKI. (f, g) Representative image of HE and PAS revealed that renal tubular injury induced by IR was partly reversed by MT and Fer-1 treatment, and renal tubular damage was scored semiquantitatively (right panel). (e) Real-time PCR and (f) immunohistochemistry were used to measure the expression of KIM-1 in kidney tissues. (i) TUNEL staining and scores. Data represent the of six mice in each group. compared to the Sham and Sham+MT (20 mg/kg) groups. compared to the IR-treated group. -100 μm.

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