MicroRNA 101 Attenuated NSCLC Proliferation through IDH2/HIFα Axis Suppression in the Warburg Effect

Figure 4

miR-101 regulated HIF1α hydroxylation and degradation. (a) HIF1α concentrations of A549 (left) and H460 (right) cells were measured by western blot after CHX treatment. (b) Statistical analysis of HIF1α concentrations of A549 (left) and H460 (right) cells measured by western blot after CHX treatment. (c) HIF1α mRNA levels were measured by RT-qPCR of A549 (left) and H460 (right) cells pretreated with α-KG or control. (d) The expression levels of HIF1α and HIF1α hydroxylation were measured by western blot analysis of A549 (up) and H460 (down) pretreated with α-KG or control. (e) HIF1α concentrations of A549 (left) and H460 (right) cells pretreated with α-KG were measured by western blot after CHX treatment. (f) Statistical analysis of HIF1α concentrations of A549 (left) and H460 (right) cells pretreated with α-KG measured by western blot after CHX treatment. , , , .

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