Dynamin-Related Protein 1 Is Involved in Mitochondrial Damage, Defective Mitophagy, and NLRP3 Inflammasome Activation Induced by MSU Crystals

Figure 9

Mito-TEMPO or Mdivi-1 pretreatment on MSU crystal-induced mouse inflammatory model produced protective effects. (a) Representative plots of migrated leukocytes (CD45⁺), neutrophils (CD11b⁺Gr-1⁺), and macrophages (CD11b⁺F4/80⁺) in peritoneal lavage fluid were detected by FCM. The number of migrated leukocytes, neutrophils, and macrophages were quantified and compared among the groups. (b) Paw swelling index. (c) The supernatants of peritoneal fluid were checked by ELISA for IL-1β. (d) HE staining of mouse footpad tissue section and representative image of microscope. for each group. (e) An immune-fluorescence assay was used to detect MPO, CD11b, and DRP1-positive cell distribution in mouse foot pad tissue sections. Blue shows nuclei stained with DAPI. mice per group. 10–12 images per mouse were analyzed. Data is . vs. Ctrl; ^# vs. MSU crystals + vehicle. All the data are expressed as the .

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