The Imbalance of Mitochondrial Homeostasis of Peripheral Blood-Derived Macrophages Mediated by MAFLD May Impair the Walking Ability of Elderly Patients with Osteopenia

Figure 6

MAFLD impairs the function of CD14 + mononuclear mitochondria in peripheral blood of osteopenia patients. (a, b) MMP and MPTP of PBMCs from the patients among OPe, OPe with MAFLD, and SO based on FCA. (c) The quantitative results of MMP and MPTP are, respectively, presented in bar plots. (d) In the heat map, the relative mRNA level of FIS1, MFF, DRP1, and MIEF1 in PBMCs from the patients among OPe, OPe with MAFLD, and SO group is determined by RT-PCR. (e) The protein level of mitochondrial fusion and division-related protein in the PBMCs from patients among OPe, OPe with MAFLD, and SO group detected by WB. (f) The protein level of mitochondrial fusion and division-related protein in the mitochondrial of PBMCs from patients among OPe, OPe with MAFLD, and SO group detected by WB. Bars, means SE; , ; , ; , ; , . Abbreviations: MMP: mitochondrial membrane potential; MPTP: mitochondrial permeability transition pore; PBMC: peripheral blood monocytes cell; SO: severe osteoporosis; OPe: osteopenia; OPe with MAFLD: osteopenia with metabolic dysfunction-associated fatty liver disease; DRP1: dynamin-related protein 1; FIS1: mitochondrial fission 1 protein; MFF: mitochondrial fission factor; MIEF1: mitochondrial elongation factor 1; MFN1: mitofusin 1; MFN2: mitofusin 2; COXIV: Cytochrome c oxidase subunit IV.

(a)

(b)

(c)

(d)

(e)

(f)