The Imbalance of Mitochondrial Homeostasis of Peripheral Blood-Derived Macrophages Mediated by MAFLD May Impair the Walking Ability of Elderly Patients with Osteopenia

Figure 7

SIRT1 defects accentuated impaired monocyte mitochondria in osteopenia patients. (a) The accumulation of lipid droplets of liver from OPe and OPe with MAFLD patients is assessed by Red Oil O staining. (b) Immunohistochemical detection of SIRT1 in the liver from OPe and OPe with MAFLD patients. The protein level of SIRT1 in liver tissue is assessed by WB. (c) The relative mRNA level of SIRT1 and TLR4 in PBMCs from the patients among OPe, OPe with MAFLD, and SO group is detected by RT-PCR. (d) The correlation matrix shows the relationship between the expression of SIRT1 and clinical indicators of patients. (e) The protein level of mitochondrial apoptosis, fusion and division-related protein is detected using WB. (f) Representative image for immunofluorescence staining shows the expression and distribution of COXIV and CYCS in PBMCs intervened by various conditioned medium. Abbreviations: SIRT1: the silent mating type information regulation 1; TLR4: toll-like receptor 4; MyD88: myeloid differentiation factor 88; BCL-2: B-cell lymphoma-2; BAX: BCL2-associated X protein; CYCS: Cytochrome C; DRP1: dynamin-related protein 1; FIS1: mitochondrial fission 1 protein; MFF: mitochondrial fission factor; MIEF1: mitochondrial elongation factor 1; MFN1: mitofusin 1; MFN2: mitofusin 2; COXIV: Cytochrome c oxidase subunit IV; PAOA: palmitic acid and oleic acid; BMI: body mass index; BMD: bone mineral density; SO: severe osteoporosis; OPe: osteopenia; OPe with MAFLD: osteopenia with metabolic dysfunction-associated fatty liver disease.

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