Research Article
Deletion of ACLY Disrupts Histone Acetylation and IL-10 Secretion in Trophoblasts, Which Inhibits M2 Polarization of Macrophages: A Possible Role in Recurrent Spontaneous Abortion
Figure 4
Knockdown of ACLY in HTR-8, JAR, and JEG-3 cells inhibited M2 polarization. (a–c) The expression of genes encoding M2 macrophage markers (CD163, CD206, and CCL18) in M0 macrophages and cocultured macrophages, as detected using qRT-PCR. (d–f) Mean fluorescence intensity (MFI) of CD206, as analyzed using flow cytometry in the three cell lines. , , compared with shCtrl+M0; ##, compared with M0.
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