Attenuation of the Severity of Acute Respiratory Distress Syndrome by Pomiferin through Blocking Inflammation and Oxidative Stress in an AKT/Foxo1 Pathway-Dependent Manner

Figure 8

Pomiferin inhibited LPS-induced inflammation and oxidative stress in RAW264.7 macrophages in an AKT/Foxo1 pathway-dependent manner. (a) Representative western blots of proteins of p-AKT and AKT in 3-DE-treated RAW264.7 macrophages (). (b–e) The mRNA levels of Tnf-α, Hmgb1, Mcp-1, and Il-1β in RAW264.7 macrophages determined by RT-qPCR (). (f–i) Representative western blots and semiquantitative results of proteins of SOD1, SOD2, and GPX4 in RAW264.7 macrophages (). (j, k) Representative western blots and semiquantitative results of proteins of p-FOXO1 and FOXO1 in RAW264.7 macrophages (). vs. the indicated groups, NS means no significance.

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