DNA Methyltransferase 3b Accelerates the Process of Atherosclerosis

Figure 1

Effects of Dnmt3b silencing on aortic root atherosclerosis lesions in ApoE^−/− mice. , . (a) Left: The fraction area of lesion was measured by Oil Red O staining, magnification ×40. Right: Quantitative analysis of aortic root AS lesion area. (b) Left: The collagen content was assessed by Sirius Red staining, magnification ×100. Right: Quantitative analysis of collagen content of plaques (3 sections per mice, ). (c) The macrophage (CD68) content was evaluated by immunofluorescence staining, magnification ×100. Right: Quantification of macrophage content in lesion by measuring the CD68-positive area within lesion (3 sections per mice, ). (d) The smooth muscle cells (α-SMA) content was evaluated by immunofluorescence staining, magnification ×100. Right: Quantitative analysis of smooth muscle cells content in lesion by measuring the α-SMA-positive area within lesion (3 sections per mice, ). Abbreviations: Dnmt3b: DNA methyltransferases 3b; WD: western diet.